Composite
Pp_22

Part:BBa_K4260012:Design

Designed by: Claudia Angélica García Alonso   Group: iGEM22_TecCEM   (2022-10-09)


Isoeugenol Monooxygenase, autoinducible coding sequence: Trp terminator, IemR, promoter, pelB, Iso a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 451
    Illegal XhoI site found at 279
    Illegal XhoI site found at 306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1199
    Illegal NgoMIV site found at 1907
    Illegal NgoMIV site found at 2515
    Illegal AgeI site found at 1731
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1135
    Illegal BsaI.rc site found at 1827


Design Notes

Since the coding sequence of IemR was in the antisense chain, the reverse-complementary sequence of the Trp terminator was used, in order to stop the transcription of the IemR.


Source

The transcriptional activator (IemR) and the promoter, come from Pseudomonas nitroreducens Jin1. The Isoeugenol monooxygenase comes from Pseudomonas putida IE27.

References